September 26, 2012 (TSR) – Researchers at the Wyss Institute for Biologically Inspired Engineering at Harvard University have created a new kind of barcode (colored fluorescent biomarkers) that could come in an almost limitless array of styles — with the potential to enable scientists to gather vastly more vital information, at one given time, than ever before.
The new method harnesses the natural ability of DNA to self-assemble.
“We hope this new method will provide much-needed molecular tools for using fluorescence microscopy to study complex biological problems,” says Peng Yin, Wyss core faculty member and study co-author who has been instrumental in the DNA origami technology at the heart of the new method.
Almost limitless color patterns
Fluorescence microscopy has been a tour de force in biomedical imaging for the last several decades. In short, scientists couple fluorescent elements — the barcodes — to molecules they know will attach to the part of the cells they wanted to investigate. Illuminating the sample triggers each kind of barcode to fluoresce at a particular wavelength of light, such as red, blue, or green — indicating where the molecules of interest are.
However, the method is limited by the number of colors available — three or four — and sometimes the colors get blurry.
That’s where the magic of the DNA barcode comes in: colored dots can be arranged into geometric patterns or fluorescent linear barcodes, and the combinations are almost limitless — substantially increasing the number of distinct molecules or cells scientists can observe in a sample, and the colors are easy to distinguish.
How it works
DNA origami follows the basic principles of the double helix in which the molecular bases A (adenosine) only bind to T (thymine), and C (cytosine) bases only bind to G (guanine).
With those “givens” in place, a long strand of DNA is programmed to self-assemble by folding in on itself with the help of shorter strands to create predetermined forms — much like a single sheet of paper is folded to create a variety of designs in the traditional oregami Japanese art.
To these more structurally complex DNA nano-structures, researchers can then attach fluorescent molecules to the desired spots, and use origami technology to generate a large pool of barcodes out of only a few fluorescent molecules. That could add a lot to the cellular imaging “toolbox” because it enables scientists to potentially light up more cellular structures than ever possible before.
“The intrinsic rigidity of the engineered DNA nanostructures is this method’s greatest advantage; it holds the fluorescent pattern in place without the use of external forces. It also holds great promise for using the method to study cells in their native environments,” Yin says. As proof of concept, the team demonstrated that one of their new barcodes successfully attached to the surface of a yeast cell.
It’s low-cost, easy to do, and more robust compared to current methods, says Yin.
The researchers plan to determine what happens when each of the fluorescent barcodes are mixed together in a cell sample, which is routine in real-life biological and medical imaging systems.
“We’re moving fast in our ability to manipulate DNA molecules using origami technology,” says Wyss Institute Founding Director Don Ingber, M.D., Ph.D., “and the landscape of its potential is tremendous — from helping us to develop targeted drug-delivery mechanisms to improving the scope of cellular and molecular activities we are able to observe at a disease site using the latest medical imaging techniques.”
The work was funded by the National Institutes of Health, the National Science Foundation, the Office of Naval Research, and the Wyss Institute.
Chenxiang Lin, Ralf Jungmann, Andrew M. Leifer, Chao Li, Daniel Levner, George M. Church, William M. Shih, Peng Yin, Submicrometre geometrically encoded fluorescent barcodes self-assembled from DNA, Nature Chemistry, 2012, DOI: 10.1038/nchem.1451